TABLE 4. Effects of intracisternally administered TRH analogs and other hypothalamic peptides on ethanol-induced sleep and hypothermia in mice.
Drug dose was 24 nmol in 10 μl of saline; doses were injected intracisternally 10 minutes after ethanol (4.7 g/kg). Rectal temperature was recorded 40 minutes after ethanol treatment.
| Treatmenta | N | Sleeping Time | Rectal Temperature |
|---|---|---|---|
| min ± S.E.M. | °C ± S.E.M. | ||
| Saline | 59 | 99.5 ± 5.1 | 35.1 ± 0.13 |
| TRH | 30 | 61.2 ± 3.6c | 37.1 ± 0.16c |
| Pyrazolyl TRH | 9 | 45.0 ± 3.6c | 37.0 ± 0.21c |
| Lysine TRH | 8 | 61.4 ± 6.1c | 36.1 ± 0.14c |
| Diiodo TRH | 19 | 65.5 ± 5.4c | 36.0 ± 0.15c |
| β-AlanineTRH | 9 | 61.8 ± 6.7c | 37.0 ± 0.19c |
| Deamidated TRH | 10 | 99.3 ± 12.9 | 36.1 ± 0.11c |
| MIF | 8 | 111.6 ± 7.9 | 35.4 ± 0.13 |
| SRIF | 16 | 90.9 ± 10.3 | 36.4 ± 0.19c |
| LHRH | 20 | 93.1 ± 8.0 | 35.5 ± 0.17 |
| Substance P | 31 | 69.1 ± 6.9b | 35.7 ± 0.20 |
| OMeDP | 20 | 105.7 ± 7.5 | 35.0 ± 0.31 |
| OMeDP + TRH | 18 | 48.1 ± 3.6d | 36.6 ± 0.19c |
TRH = pyroGlu-His-Pro-NH2; pyrazolyl TRH = pyroGlu-(pyrazolyl-3) Ala-Pro-NH2; lysine TRH = pyroGlu-Lys-Pro-NH2; β-alanine TRH = pyroGlu-His-Pro-βAla-NH2; diiodo TRH = pyroGlu-2,4-diiodo-His-Pro-NH2; deamidated TRH = pyroGlu-His-Pro; MW = Pro-Leu-Gly-NH2; SRIF = Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys-OH; LHRH = pyroGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2; substance P = Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2; OMeDP = pyro-Glu-His-OME.
When compared with saline, P < .01.
When compared with saline, P < .001.
When compared with TRH alone, P < .02.