TABLE 6. Effects of noradrenergic agonists on ethanol-induced sleep and noradrenergic antagonists on TRH reduction of ahanol-induced sleep in mice.

All mice received 4.6 g/kg of ethanol 10 minutes before intracisternal drug treatments. Each group contained 8 to 60 mice. α-Methyltyrosine, ethanol and TRH were administered intraperitoneally; all other drugs were injected intracisternally and are reported as total dose per brain. Rectal temperature was measured 40 minutes after ethanol administration. When pretreatment groups (Saline, α-methyltyrosine, propranolol, phentolamine) are compared to corresponding group plus TRH, a percent sleep reduction by TRH is obtained. These values are: saline + TRH = 29.5 + 4.2%; a-methyltyrosine + TRH = 50.7 ± 6.6% (P < .05); 35 μg of propranolol + TRH = 31.4 ± 6.6%; phentolamine + TRH = 58.4 + 5.2% (P < .001). When similar comparisons are made for percent antagonism of hypothermia, the values are: saline + TRH = 4.3 ± 0.47%; α-methyltyrosine + TRH = 3.9 ± 0.59%; 35 μg of propranolol + TRH = 2.2 ± 0.38% (P < .005); phentolamine + TRH = 1.0 ± 0.33% (P < .001)

Treatment	Dose	Sleeping Time	Rectal Temperature
		min ± S.E.M.	°C ± S.E.M.
A. Noradrenergic agonists
Saline		91.0 ± 4.8	35.6 ± 0.14
l-Norepinephrine	0.2 μg	103.6 ± 7.1	35.6 ± 0.26
	5 μg	113.9 ± 14.3	37.3 ± 0.17d
	30 μg	150.0 ± 15.0c	35.5 ± 0.27
Xylometazoline	0.2 μg	138.9 ± 17.9	34.5 ± 0.29a
	5 μg	109.4 ± 17.3	35.0 ± 0.19
	20 μg	132.6 ± 13.5	34.8 ± 0.17b
d-Amphetamine	4 μg	91.4 ± 9.5	35.1 ± 0.33
	8 μg	80.6 ± 10.8	36.8 ± 0.45
	50 μg	76.6 ± 7.9	37.3 ± 0.32d
	100 μg	93.2 ± 8.9	38.2 ± 0.24d
B. Noradrenergic antagonists and TRH
Saline		82.8 ± 4.4	35.6 ± 0.10
TRH	10 mg/kg	51.8 ± 35c	37.1 ± 0.17d
α-Methyltyrosine	300 mg/kg	83.0 ± 9.5	35.5 ± 0.21
α-Methyltyrosine + TRH	300 mg/kg + 10 mg/kg	40.9 ± 55e	37.0 ± 0.21g
dl-Propranolol	5 μg	98.5 + 12.3
	10 μg	63.3 ± 5.3d	35.1 ± 0.21
	20 μg	77.8 ± 5.1	34.6 ± 0.20d
	35 μg	83.3 ± 7.2	35.1 ± 0.19
dl-Propranolol + TRH	10 μg + 10 mg/kg	40.6 ± 3.8g	36.4 ± 0.19g
	20 μg + 10 mg/kg	55.6 ± 5.8f	35.7 ± 0.28g
	35 μg + 10 mg/kg	60.3 ± 5.6e	35.8 ± 0.12g
Phentolamine	2 μg	147.4 ± 19.3d	35.5 ± 0.11
Phentolamine + TRH	2 μg + 10 mg/kg	61.7 ± 77g	36.0 ± 0.12f

When compared with saline:

^aP < .05.

^bP < .025.

^cP < .005.

^dP < .001.

When compared with treated group without TRH:

^eP < .02.

^fP < .01.

^gp < .005.