TABLE 8. Effects of drugs which affect cholinergic mechanisms on ethanol-induced sleep and sleep reduction produced by TRH.

All mice received 4.7 g/kg of ethanol. TRH (10 mg/kg) was administered intraperitoneally 10 minutes after ethanol. All other compounds including atropine methyl nitrate (AMN) were administered intracisternally (i.e.) 10 minutes after ethanol.

Treatment	Dose	N	Sleeping Time
			min ± S.E.M.
Saline		47	104.7 ± 5.0
TRH	10 mg/kg	36	56.6 ± 4.0b
Atropine methyl nitrate (AMN)	10 μg	19	95.8 ± 6.2
d-Tubocurarine	5 μg	19	32.5 ± 3.1b
d-Tubocurarine + TRH	5 μg + 10 mg/kg	9	20.8 ± 1.9b,e
d-Tubocurarine	3 μ	9	51.9 ± 4.7b
d-Tubocurarine + AMN	3 μg + 10 μg	9	44.2 ± 2.6b
Gallamine	5 μg	18	43.7 ± 3.3b
Gallamine + TRH	5 μg + 10mg/kg	9	28.1 ± 1b,e
Gallamine	3 μg	10	56.4 ± 7.0b
Gallamine + AMN	3 μg + 10 μg	10	70.2 ± 8.6a
Hexamethonium	l00 μg	20	76.7 ± 7.2a
Hexamethonium + TRH	100 μg + 10 mg/kg	18	49.9 ± 6.4a,d
Hexamethonium + AMN	l00 μg + 10 μg	10	95.6 + 14.04
Carbachol	0.3 μg	28	74.9 ± 4.9b
Carbachol + TRH	0.3 μg + 10mg/kg	20	103.1 ± 11.7
Carbachol + AMN	0.3 μg + l0 μg	20	93.2 ± 5.5c

When compared with saline:

^aP < .02.

^bP < .001.

When compared with appropriate group that received only d-tubocurarine, gallamine, hexamethonium or carbachol:

^cP < 0.5

^dP < .01.

^eP < .001.