Table 1.
Accession # | Primer name: Primer sequence | Position on cDNA (Relative to A of ATG) |
---|---|---|
U16126 | F1: 5′ATGAAGATTATTTTCCCGATTC3′ | +1 to +22 |
F2: 5′TTCCAAGCCCTTTATGACAC3′ | +1587 to +1606 | |
F3: 5′GAACTAATTGATCATAAATCAAAAATC3′ | +1510 to +1536 | |
R1: 5′TTATGCCATGGTTTCTTTACC3′ | +2727 to +2707 | |
R2: 5′CTACAGAAGGACCTCTTTTCCAATTG3′ | +2576 to +2551 | |
R3: 5′GACAACACTGTAGAAATGGAA3′ | +2639 to +2619 | |
R4: 5′CTGGATGGTTATGGTGGCACT3′ | +2599 to +2580 | |
R5: 5′TCATATTCAGGCCACTGGCT3′ | +1240 to +1221 | |
R6: 5′GTGGTTCCTTGAGAATATCC3′ | +98 to +78 | |
Accession # | Primer Name: Primer sequence | Position on genomic DNA (relative to A of ATG) |
AP002528 | F4: 5′ CAAGAGGCGAGGAACCCCCGC3′ | −5984 to −5964 |
F5: 5′ TTCTCTCGCTCTAGCTCCT3′ | −5772 to −5754 | |
F6: 5′ AAAGCCATCACCCAGAAGCAA3′ | −5664 to −5644 | |
F7: 5′ ACCAAGGCTGGGTTTCCTAC3′ | −341 to −322 | |
F8: 5′ CGAGGGCCCATTAGGCTGCA3′ | −7500 to −7480 | |
F9: 5′ ATCTTGGAGTCTGCGACCT3′ | −6443 to −6425 | |
F10: 5 AAAGCCATCACCCAGAAGCAA3′ | −5984 to −5966 | |
F11: 5′ CTTAGTCTCCCCATCCCAGGA3′ | −5863 to −5842 | |
F12: 5′ GGGAGCGAAGTCCAGCCGGAGGC3′ | −5827 to −5805 | |
F13: 5′ ATTCTCTCGCTCTAGCTCCTGTG3′ | −5773 to −5751 | |
F14: 5′ CTCCTCGGTCACCGTCTTCGCC3′ | −5601 to −5580 | |
F15: 5′ TTCCGCCCGACCTCCTCCG3′ | −5518 to −5500 | |
F16: 5′ TAAGGTGCTCGAACAGTCG3′ | −1839 to −1821 | |
F17: 5′ GAATAAACTCCTAACGAAAGCC3′ | −1257 to −1236 | |
F18: 5′ AAACTGACTTTGATTTAGCTAG3′ | −710 to −688 | |
F19: 5′ ACCAAGGCTGGGTTTCCTAC3′ | −341 to −322 | |
F20: 5′ ATGAAGATTATTTTCCCGAT3′ | −1 to +20 | |
R7: 5′TGACGGCAGCGGCGAAGTC3′ | −5571 to −5589 | |
R8: 5′AGCTAGAGCGAGAGAATTC3′ | −5757 to −5775 | |
R9: 5′ AAGACTCGCAGTGAATCCGT3′ | −5253 to −5272 | |
R10: 5′ GGTAAAGAAACTCTATCTCCT3′ | −4820 to −4840 | |
R11: 5′ AGAGCGAACCGCCTGTTTA3′ | +170 to +188 |
The nucleotide A of ATG codon is designated as +1, and bases upstream of +1 in the genomic sequence are given negative numbers.