Table 2.
Primer sequences for HOXB1 gene PCR amplification and DHPLC analysis.
| Exon | Primer name | Primer sequences (5'-3') | Amplicon size (bp) | PCR annealing temp (°C) | DHPLC oven temp (°C) |
|---|---|---|---|---|---|
| 1 | HOXB1-F1 for | CATACTGCCGAAAGGTTGTAG | 365 | 60 | 61.5, 65.6, 66.2 |
| HOXB1-F1 rev | TAGTACTGAGAAGGCCCGTA | ||||
| 1 | HOXB1-F2 for | GGTATGCTCCTGCCGCCTGCA | 227 | 58 | 63.3, 65.5 |
| HOXB1-F2 rev | ATCAGCATAGGCCGGTGCAA | ||||
| 1 | HOXB1-F3 for | AGCATCCCCCTTATGGGAA | 282 | 58 | 62.8 |
| HOXB1-F3 rev | CTTACCTGTGTCTACCAGAG | ||||
| 2 | HOXB1-F4 for | GAGAATTGACCTGGCCTTTC | 359 | 60 | 62.9, 63.4 |
| HOXB1-F4 rev | TGACAGAGCTGGGTGAGGCTT | ||||
| 2 | HOXB1-F5 for | TTTGGTTCCAGAACCGACGA | 300 | 60 | 64.0, 65.5 |
| HOXB1-F5 rev | GGCAGCTCTAAACTGGACTT | ||||
PCR, polymerase chain reaction; DHPLC, denaturing high performance liquid chromatography; temp, temperature.