Table 3.
Primer sequence for HOXB1 SNP genotyping.
| Exon | Primer name | Primer sequences (5'-3') | Amplicon size (bp) | PCR annealing temp (°C) |
|---|---|---|---|---|
| 1 | HOXB1-F1 for | CATACTGCCGAAAGGTTGTAG | 365 | 60 |
| HOXB1-F1 rev | TAGTACTGAGAAGGCCCGTA | |||
| 1 | HOXB1-F2 for | GGTATGCTCCTGCCGCCTGCA | 227 | 58 |
| HOXB1-F2 rev | ATCAGCATAGGCCGGTGCAA | |||
| 1 | IVS1+63 G/A-mut_for | AAAGCATCTCTGCTTCCCCTGCGG | 118 | 58 |
| IVS1+63G/A_rev | GACCTCACCTGACCTGAGAC | |||
| 2 | S291N-mut_for | ACCTGAGCCGGGCCCGGAGGATG | 366 | 58 |
| HOXB1-F5 rev | GGCAGCTCTAAACTGGACTT | |||
PCR, polymerase chain reaction; temp, temperature.