Table 1.
PCR primer pairs used in this study
| Sequences | Position | Gen Bank | |||||
|---|---|---|---|---|---|---|---|
| 18S forward | 5' | TCA ACT TTC GAT GGT AGT CGC CGT | 3' | 363 | - | 386 | X01117 |
| 18S reverse | 5' | TCC TTG GAT GTG GTA GCC GTT TCT | 3' | 470 | - | 447 | |
| B1 receptor forward | 5' | GCA GCG CTT AAC CAT AGC GGA AAT | 3' | 367 | - | 391 | NM_030851 |
| B1 receptor reverse | 5' | CCA GTT GAA ACG GTT CCC GAT GTT | 3' | 478 | - | 454 | |
| IL-1β forward | 5' | TGT CAC TCA TTG TGG CTG TGG AGA | 3' | 247 | - | 270 | NM_031512 |
| IL-1β reverse | 5' | TGG GAA CAT CAC ACA CTA GCA GGT | 3' | 411 | - | 388 | |
| TNF-α forward | 5' | ATG ATC CGA GAT GTG GAA CTG GCA | 3' | 160 | - | 183 | NM_012675 |
| TNF-α reverse | 5' | AAT GAG AAG AGG CTG AGG CAC AGA | 3' | 257 | - | 234 | |
| TRPV1 forward | 5' | GCA CAA TGG GCA GAA TGA CAC CAT | 3' | 575 | - | 598 | NM_031982 |
| TRPV1 reverse | 5' | GGC ATT GAC AAA CTG CTT CAG GCT | 3' | 656 | - | 633 | |