Skip to main content
. 2010 Jul 10;29(1):92. doi: 10.1186/1756-9966-29-92

Figure 3.

Figure 3

TLR4 expression and functional effect after TLR4 knockdown in human breast cancer cell line MDA-MB-231. A, immunofluorescence analysis of gene-specific siRNA on TLR4 protein expression in pGenesil-1 vector, ScrambledsiRNA and TLR4AsiRNA transfected cells. Nuclear staining was performed using DAPI (blue) (200×). B, MTT analysis of the proliferative rate of pGenesil-1 vector, ScrambledsiRNA and TLR4AsiRNA transfected cells. C and D, IL-6 and IL-8 presence in the supernatant secreted by pGenesil-1 vector, ScrambledsiRNA and TLR4AsiRNA transfected cells. Cell supernatant was analyzed using flow cytometry. All results are representative of three separate experiments.