Table 1.

PCR primers of human TLRs

Genes	Primer sequence (5'-3')	Amplification size (bp)
TLR1	For: TCTGGTACACGCATGGTC	517 bp
	Rev: ATGGGTGGGAAACTGAAT
TLR2	For: AACTTACTGGGAAATCCTTAC	264 bp
	Rev: AAAAATCTCCAGCAGTAAAAT
TLR3	For: GCATTTGTTTTCTCACTCTTT	131 bp
	Rev: TTAGCCACTGAAAAGAAAAAT
TLR4	For: CGAGGAAGAGAAGACACCAGT	106 bp
	Rev: CATCATCCTCACTGCTTCTGT
TLR5	For: AGCTTCAACTATATCAGGACA	383 bp
	Rev: TGGTTGGAGGAAAAATCTAT
TLR6	For: CTTCCATTTTGTTTGCCTTAT	123 bp
	Rev: AGCGGTAGGTCTTTTGGAAC
TLR7	For: AAACTCCTTGGGGCTAGATG	149 bp
	Rev: AGGGTGAGGTTCGTGGTGTT
TLR8	For: CTGTGAGTTATGCGCCGAAGA	246 bp
	Rev: TGGTGCTGTACATTGGGGTTG
TLR9	For: CGCCCTGCACCCGCTGTCTCT	168 bp
	Rev: CGGGGTGCTGCCATGGAGAAG
TLR10	For: AGAAGAAAGGGAACTGATGAC	279 bp
	Rev: CCTGCCAGTAAATACCAAGT
GAPDH	For: GGATTTGGTCGTATTGGG	205 bp
	Rev: GGAAGATGGTGATGGGATT