Table 1.
The primer sequences, exons amplified and the restriction enzymes used in the PCR/RFLP procedures to detect five G6PD deficient mutations and the silent one [20-23]
| Variant | Primers | Exon amplified | Restriction Enzyme |
|---|---|---|---|
| Mediterranean (563 C→T) |
5'GGT GAG GCT CCT GAG TAC CA 3' | VI | MboII |
| 5'AGC TGT GAT CCT CAC TCC CC 3' | |||
| Chatham (1003 G→A) |
5'CAA GGA GCC CAT TCT CTC CCT T 3' | IX | BstXI |
| 5'TTC TCC ACA TAG AGG AGG ACG GCT GCC AAA GT3' | |||
| A- (202 G→A) |
5'CGT GTC CCC AGC CAC TTC TA 3' | III-V | NlaIII |
| 5' CAC GCT CAT AGA GTG GTG GG 3' | |||
| Cosenza (1376 G→C) |
5'GCA GCC AGT GGG ATC AGC AAG 3' 5' GGC AAG GAG GGT GGC CGT GG 3' |
XI-XIII | Bsu361 |
| Aures (143 T→C) |
5' CAG CCA CTT CTA ACC ACA CAC Ct 3' 5' CCG AAG TTG GCC ATG CTG GG 3' |
III | Mbo I |
| Silent (1311 C→T) |
5' TGT TCT TTC AAC CCC GAG GAG T 3' 5' AAG ACG TCC AGG ATG AGG TGA TC 3' |
Part X-XI | Bcl I |