Table 1.
Clonagenicity of the epithelial cell lines with dnLMP1
| cell line | transgene | colonies per μg of DNA | colony ratio | |
|---|---|---|---|---|
| status | pGFPdnLMP1 | pGFP | pGFPdnLMP1:pGFP | |
| 53.217 | - | 164 | 170.4 | 1 : 1.0 |
| 53.234a | + | 8.8 | 41.6 | 1 : 4.7 |
| 53.204 | + | 0 | 4.2 | - |
| 53.191 | + | 14 | 42 | 1 : 3.0 |
| 53.226b | + | 1.6 | 6 | 1 : 3.8 |
| 53.278a | + | 46 | 112 | 1 : 2.4 |
| 53.278b | + | 1.2 | 13.2 | 1 : 11.0 |
The various cell lines (as shown) were transfected and grown for two weeks under G418 selection before fixing and staining with crystal violet (details in the legend to figure 2). The number of colonies per μg of DNA and the ratio of colonies for the GFPdnLMP1:GFP plasmids are tabulated.