Table 1.
Panel of antibodies selected in the study
| Antibody against | Pre--treatment | Incubation time of primary antibody, temperature | Concentration of primary antibody |
|---|---|---|---|
| GFAP (Dako, Copenhagen, Denmark) | No pre-treatment | 120 min, 20°C | 1:300 |
| TNFα (Santa Cruz, CA, USA) | boiling in 0.1 M Citric Acid buffer. | 120 min, 20°C | 1:600 |
| IL-1β (Santa Cruz, CA, USA) | boiling in 0.25 mM EDTA buffer. | 120 min, 20°C | 1:4000 |
| IL-6 (Santa Cruz, CA, USA) | 5 min Proteolytic Enzyme (Dako, Copenhagen, Denmark), 20°C. | 120 min, 20°C | 1:2000 |
| CD68 (Serotec, United Kingdom) | 5 min Proteolytic Enzyme (Dako, Copenhagen, Denmark), 20°C. | 120 min, 20°C | 1:200 |
| HSP 27 (NovaCastra, United Kingdom) | boiling in 0.25 mM EDTA buffer.. | 120 min, 20°C | 1:20 |
| HSP 70 (NovaCastra, United Kingdom) | boiling in 0.25 mM EDTA buffer.. | Overnight, 20°C | 1:100 |
| HSP 90 (NovaCastra, United Kingdom) | boiling in 0.25 mM EDTA buffer.. | 120 min, 20°C | 1:50 |
| COX2 (Santa Cruz, CA, USA) | 5 min Proteolytic Enzyme (Dako, Copenhagen, Denmark), 20°C. | 120 min, 20°C | 1:100 |
| ORP-150 (IBL, Gunma, Japan) | boiling in 0.1 M Citric Acid buffer. | 120 min, 20°C | 1:200 |
| β-APP (NovaCastra, United Kingdom) | boiling in 0.1 M Citric Acid buffer. | Overnight, 20°C | 1:300 |
| TrypH (NovaCastra, United Kingdom) | boiling in 0.1 M Citric Acid buffer. | Overnight, 20°C | 1:50 |
| GAP-43 (Santa Cruz, CA, USA) | boiling in 0.1 M Citric Acid buffer | 120 min, 20°C | 1:2000 |
| TdT enzyme (Chemicon, CA, USA) | 15 min in Proteinase K (20 μg/ml), 20°C | 60 min, 38°C | 30 μl of TdT in 70 μl of reaction buffer (Chemicon, CA, USA) |