Figure 3.

Percent change in the number of cisplatin-induced Tunel-positive OvCa cells. OVCAR-3 and SKOV-3 cells were cultured for 24 hours with 5 μg/ml cisplatin or with 0 or 100 ng/ml of CCL25 plus 1 μg/ml of anti-human CCR9 or isotype control antibodies. Detection of apoptotic cells was carried out using the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) method. Apoptotic cells exhibited nuclear green fluorescence with a standard fluorescence filter set (520 ± 20 nm). Asterisks (*) indicate statistical significant differences (p < 0.01) between treated and untreated OvCa cells.