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Coffea arabica transformation steps. A) Friable callus used in the transformation. (B) Callus after bombardment under kanamycin selection. (C) Regenerating embryo. (D) Small coffee plant under kanamycin selection. (E) Plant transferred to the greenhouse. (F) Coffee plants positive for the α-AI1 gene. (G) Flowers of the transformed coffee plant. (H) Transgenic fruits, 10 weeks after flowering. (I) T1 generation from plant 3.
