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Southern blot indicating the presence of α-AI1 in the genome of Coffea arabica T0 plants. P, positive control (α-AI1 Fragment, 735 bp); N, non-transformed coffee plant; 1 - 6, α-AI1 positive coffee plants (4150-bp fragment). Plant DNA extracts were digested with the enzymes PvuII, EcoRI and ClaI at the same time.
