Table 1.
ABC and SLC genes included on the RT2 Profiler™ Array and their relative hCMEC/D3 Expression.
| Unigene | GeneBank | Symbol | Description | Gene Name | hCMEC/D3 Relative Expression [Mean ΔCt × 10−3 ± (S.D.)] |
|---|---|---|---|---|---|
| Hs.429294 | NM_005502 | ABCA1 | ABC, sub-family A (ABC1), member 1 | ABC-1/ABC1 | 4.829 (0.34) |
| Hs.134585 | NM_173076 | ABCA12 | ABC, sub-family A (ABC1), member 12 | DKFZp434G232/ICR2B | 0.069 (0.04) |
| Hs.226568 | NM_152701 | ABCA13 | ABC, sub-family A (ABC1), member 13 | DKFZp313D2411 | 0.005 (0.00) |
| Hs.421202 | NM_001606 | ABCA2 | ABC, sub-family A (ABC1), member 2 | ABC2 | 2.916 (0.16) |
| Hs.26630 | NM_001089 | ABCA3 | ABC, sub-family A (ABC1), member 3 | ABC-C/ABC3 | 7.465 (0.94) |
| Hs.416707 | NM_000350 | ABCA4 | ABC, sub-family A (ABC1), member 4 | ABC10/ABCR | 0.321 (0.08) |
| Hs.131686 | NM_080283 | ABCA9 | ABC, sub-family A (ABC1), member 9 | DKFZp686F2450/EST640918 | 0.195 (0.05) |
| Hs.489033 | NM_000927 | ABCB1 | ABC, sub-family B (MDR/TAP), member 1 | ABC20/CD243 | 58.029 (7.96) |
| Hs.658439 | NM_003742 | ABCB11 | ABC, sub-family B (MDR/TAP), member 11 | ABC16/BRIC2 | 0.039 (0.01) |
| Hs.654403 | NM_000443 | ABCB4 | ABC, sub-family B (MDR/TAP), member 4 | ABC21/GBD1 | 0.059 (0.01) |
| Hs.658821 | NM_178559 | ABCB5 | ABC, sub-family B (MDR/TAP), member 5 | ABCB5alpha/ABCB5beta | No Ct |
| Hs.107911 | NM_005689 | ABCB6 | ABC, sub-family B (MDR/TAP), member 6 | ABC/ABC14 | 8.131 (1.00) |
| Hs.709181 | NM_004996 | ABCC1 | ABC, sub-family C (CFTR/MRP), member 1 | ABC29/ABCC | 31.571 (6.02) |
| Hs.55879 | NM_033450 | ABCC10 | ABC, sub-family C (CFTR/MRP), member 10 | EST182763/MRP7 | 3.562 (0.31) |
| Hs.652267 | NM_032583 | ABCC11 | ABC, sub-family C (CFTR/MRP), member 11 | EWWD/MRP8 | No Ct |
| Hs.410111 | NM_033226 | ABCC12 | ABC, sub-family C (CFTR/MRP), member 12 | MRP9 | 0.031 (0.00) |
| Hs.368243 | NM_000392 | ABCC2 | ABC, sub-family C (CFTR/MRP), member 2 | ABC30/CMOAT | 0.420 (0.05) |
| Hs.463421 | NM_003786 | ABCC3 | ABC, sub-family C (CFTR/MRP), member 3 | ABC31/DKFZp686E22157 | 4.840 (0.78) |
| Hs.508423 | NM_005845 | ABCC4 | ABC, sub-family C (CFTR/MRP), member 4 | EST170205/MOAT-B | 26.918 (4.02) |
| Hs.368563 | NM_005688 | ABCC5 | ABC, sub-family C (CFTR/MRP), member 5 | ABC33/DKFZp686C1782 | 9.798 (0.07) |
|
| |||||
| Hs.442182 | NM_001171 | ABCC6 | ABC, sub-family C (CFTR/MRP), member 6 | ABC34/ARA | 0.090 (0.03) |
| Hs.159546 | NM_000033 | ABCD1 | ABC, sub-family D (ALD), member 1 | ABC42/ALD | 2.971 (0.51) |
| Hs.700576 | NM_002858 | ABCD3 | ABC, sub-family D (ALD), member 3 | ABC43/PMP70 | 64.739 (7.87) |
| Hs.94395 | NM_005050 | ABCD4 | ABC, sub-family D (ALD), member 4 | ABC41/EST352188 | 0.250 (0.10) |
| Hs.655285 | NM_001090 | ABCF1 | ABC, sub-family F (GCN20), member 1 | ABC27/ABC50 | 46.211 (9.71) |
| Hs.480218 | NM_004827 | ABCG2 | ABC, sub-family G (WHITE), member 2 | ABC15/ABCP | 2.401 (0.52) |
| Hs.413931 | NM_022437 | ABCG8 | ABC, sub-family G (WHITE), member 8 | GBD4/STSL | 0.063 (0.02) |
| Hs.76152 | NM_198098 | AQP1 | Aquaporin 1 (Colton blood group) | AQP-CHIP/CHIP28 | 0.136 (0.04) |
| Hs.455323 | NM_001170 | AQP7 | Aquaporin 7 | AQP7L/AQP9 | 0.087 (0.01) |
| Hs.104624 | NM_020980 | AQP9 | Aquaporin 9 | HsT17287/SSC1 | No Ct |
|
| |||||
| Hs.389107 | NM_001694 | ATP6V0C | ATPase, H+ transporting, lysosomal 16kDa, V0 sub c | ATP6C/ATP6L | 83.087 (16.93) |
| Hs.496414 | NM_000052 | ATP7A | ATPase, Cu++ transporting, alpha polypeptide | MK/MNK | 8.677 (0.46) |
| Hs.492280 | NM_000053 | ATP7B | ATPase, Cu++ transporting, beta polypeptide | PWD/WC1 | 1.794 (0.30) |
| Hs.632177 | NM_017458 | MVP | Major vault protein | LRP/VAULT1 | 63.929 (9.04) |
| Hs.952 | NM_003049 | SLC10A1 | SLC 10 (sodium/bile acid cotransporter family), member 1 | NTCP | 0.161 (0.14) |
| Hs.194783 | NM_000452 | SLC10A2 | SLC 10 (sodium/bile acid cotransporter family), member 2 | ASBT/ISBT | No Ct |
| Hs.436893 | NM_005073 | SLC15A1 | SLC 15 (oligopeptide transporter), member 1 | HPECT1/HPEPT1 | 0.052 (0.00) |
| Hs.518089 | NM_021082 | SLC15A2 | SLC 15 (H+/peptide transporter), member 2 | PEPT2 | 0.088 (0.05) |
| Hs.75231 | NM_003051 | SLC16A1 | SLC 16, member 1 (monocarboxylic acid transporter 1) | HHF7/MCT | 10.736 (0.67) |
| Hs.75317 | NM_006517 | SLC16A2 | SLC 16, member 2 (monocarboxylic acid transporter 8) | AHDS/DXS128 | 0.067 (0.01) |
|
| |||||
| Hs.500761 | NM_004207 | SLC16A3 | SLC 16, member 3 (monocarboxylic acid transporter 4) | MCT3/MCT4 | 36.966 (7.17) |
| Hs.84190 | NM_194255 | SLC19A1 | SLC 19 (folate transporter), member 1 | CHMD/FOLT | 3.046 (0.16) |
| Hs.30246 | NM_006996 | SLC19A2 | SLC 19 (thiamine transporter), member 2 | TC1/THT1 | 10.741 (0.77) |
| Hs.221597 | NM_025243 | SLC19A3 | SLC 19, member 3 | THTR2 | 0.026 (0.00) |
| Hs.117367 | NM_003057 | SLC22A1 | SLC 22 (organic cation transporter), member 1 | HOCT1/OCT1 | 0.103 (0.11) |
| Hs.436385 | NM_003058 | SLC22A2 | SLC 22 (organic cation transporter), member 2 | OCT2 | No Ct |
| Hs.567337 | NM_021977 | SLC22A3 | SLC 22 (extraneuronal monoamine transporter), member 3 | EMT/EMTH | 0.784 (0.24) |
| Hs.369252 | NM_004790 | SLC22A6 | SLC 22 (organic anion transporter), member 6 | HOAT1/OAT1 | No Ct |
| Hs.485438 | NM_006672 | SLC22A7 | SLC 22 (organic anion transporter), member 7 | NLT/OAT2 | No Ct |
| Hs.266223 | NM_004254 | SLC22A8 | SLC 22 (organic anion transporter), member 8 | OAT3 | No Ct |
|
| |||||
| Hs.502772 | NM_080866 | SLC22A9 | SLC 22 (organic anion transporter), member 9 | HOAT4/OAT4 | No Ct |
| Hs.459187 | NM_004213 | SLC28A1 | SLC 28 (sodium-coupled nucleoside transporter), member 1 | CNT1/HCNT1 | No Ct |
| Hs.367833 | NM_004212 | SLC28A2 | SLC 28 (sodium-coupled nucleoside transporter), member 2 | CNT2/HCNT2 | 0.082 (0.03) |
| Hs.591877 | NM_022127 | SLC28A3 | SLC 28 (sodium-coupled nucleoside transporter), member 3 | CNT3 | No Ct |
| Hs.25450 | NM_004955 | SLC29A1 | SLC 29 (nucleoside transporters), member 1 | ENT1 | 40.233 (11.21) |
| Hs.569017 | NM_001532 | SLC29A2 | SLC 29 (nucleoside transporters), member 2 | DER12/ENT2 | 1.101 (0.33) |
| Hs.473721 | NM_006516 | SLC2A1 | SLC 2 (facilitated glucose transporter), member 1 | DYT17/DYT18 | 89.189 (12.62) |
| Hs.167584 | NM_000340 | SLC2A2 | SLC 2 (facilitated glucose transporter), member 2 | GLUT2 | 0.044 (0.00) |
| Hs.419240 | NM_006931 | SLC2A3 | SLC 2 (facilitated glucose transporter), member 3 | GLUT3 | 7.200 (0.77) |
| Hs.532315 | NM_001859 | SLC31A1 | SLC 31 (copper transporters), member 1 | COPT1/CTR1 | 37.374 (3.81) |
|
| |||||
| Hs.221847 | NM_018976 | SLC38A2 | SLC 38, member 2 | ATA2/PRO1068 | 259.569 (43.26) |
| Hs.195155 | NM_033518 | SLC38A5 | SLC 38, member 5 | JM24/SN2 | 5.471 (0.41) |
| Hs.112916 | NM_000341 | SLC3A1 | SLC 3 (cystine, dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1 | ATR1/CSNU1 | 32.043 (2.43) |
| Hs.502769 | NM_002394 | SLC3A2 | SLC 3 (activators of dibasic and neutral amino acid transport), member 2 | 4F2/4F2HC | 128.694 (16.80) |
| Hs.1964 | NM_000343 | SLC5A1 | SLC 5 (sodium/glucose cotransporter), member 1 | D22S675/NAGT | 0.025 (0.00) |
| Hs.130101 | NM_014227 | SLC5A4 | SLC 5 (low affinity glucose cotransporter), member 4 | SAAT1/SGLT3 | 0.229 (0.01) |
| Hs.489190 | NM_014251 | SLC25A13 | SLC 25, member 13 (citrin) | ARALAR2/CITRIN | 19.461 (2.26) |
| Hs.390594 | NM_014331 | SLC7A11 | SLC 7, (cationic amino acid transporter, y+ system) member 11 | CCBR1/xCT | 12.157 (0.64) |
| Hs.513797 | NM_003486 | SLC7A5 | SLC 7 (cationic amino acid transporter, y+ system), member 5 | 4F2LC/CD98 | 29.632 (3.41) |
| Hs.653193 | NM_003983 | SLC7A6 | SLC 7 (cationic amino acid transporter, y+ system), member 6 | DKFZp686K15246/LAT-2 | 26.364 (5.09) |
|
| |||||
| Hs.513147 | NM_003982 | SLC7A7 | SLC 7 (cationic amino acid transporter, y+ system), member 7 | LAT3/LPI | 0.879 (0.24) |
| Hs.632348 | NM_182728 | SLC7A8 | SLC 7 (cationic amino acid transporter, y+ system), member 8 | LAT2/LPI-PC1 | 0.092 (0.02) |
| Hs.408567 | NM_014270 | SLC7A9 | SLC 7 (cationic amino acid transporter, y+ system), member 9 | BAT1/CSNU3 | 0.031 (0.00) |
| Hs.46440 | NM_005075 | SLCO1A2 | Solute carrier organic anion transporter family, member 1A2 | OATP/OATP-A | No Ct |
| Hs.449738 | NM_006446 | SLCO1B1 | Solute carrier organic anion transporter family, member 1B1 | LST-1/LST1 | No Ct |
| Hs.504966 | NM_019844 | SLCO1B3 | Solute carrier organic anion transporter family, member 1B3 | LST-3TM13/LST3 | No Ct |
| Hs.518270 | NM_005630 | SLCO2A1 | Solute carrier organic anion transporter family, member 2A1 | OATP2A1/PGT | 14.307 (1.98) |
| Hs.7884 | NM_007256 | SLCO2B1 | Solute carrier organic anion transporter family, member 2B1 | DKFZp686E0517/OATP-B | 0.318 (0.08) |
| Hs.311187 | NM_013272 | SLCO3A1 | Solute carrier organic anion transporter family, member 3A1 | OATP-D/OATP3A1 | 0.756 (0.19) |
| Hs.235782 | NM_016354 | SLCO4A1 | Solute carrier organic anion transporter family, member 4A1 | OATP-E/OATP1 | 6.245 (1.07) |
|
| |||||
| Hs.352018 | NM_000593 | TAP1 | Transporter 1, ABC, sub-family B (MDR/TAP) | ABC17/ABCB2 | 5.281 (0.70) |
| Hs.502 | NM_000544 | TAP2 | Transporter 2, ABC, sub-family B (MDR/TAP) | ABC18/ABCB3 | 48.582 (6.78) |
| Hs.519320 | NM_003374 | VDAC1 | Voltage-dependent anion channel 1 | PORIN/PORIN-31-HL | 163.857 (5.33) |
| Hs.355927 | NM_003375 | VDAC2 | Voltage-dependent anion channel 2 | FLJ23841 | 304.554 (30.63) |
| Hs.534255 | NM_004048 | B2M | Beta-2-microglobulin | B2M | Housekeeper 1 |
| Hs.412707 | NM_000194 | HPRT1 | Hypoxanthine phosphoribosyltransferase 1 | HGPRT/HPRT | Housekeeper 2 |
| Hs.523185 | NM_012423 | RPL13A | Ribosomal protein L13a | RPL13A | Housekeeper 3 |
| Hs.592355 | NM_002046 | GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | G3PD/GAPD | Housekeeper 4 |
| Hs.520640 | NM_001101 | ACTB | Actin, beta | PS1TP5BP1 | Housekeeper 5 |
| N/A | SA_00105 | HGDC | Human Genomic DNA Contamination | HIGX1A | N/A |
|
| |||||
| N/A | SA_00104 | RTC | Reverse Transcription Control | RTC | N/A |
| N/A | SA_00104 | RTC | Reverse Transcription Control | RTC | N/A |
| N/A | SA_00104 | RTC | Reverse Transcription Control | RTC | N/A |
| N/A | SA_00103 | PPC | Positive PCR Control | PPC | N/A |
| N/A | SA_00103 | PPC | Positive PCR Control | PPC | N/A |
| N/A | SA_00103 | PPC | Positive PCR Control | PPC | N/A |