. Author manuscript; available in PMC: 2011 Aug 1.

Published in final edited form as: Mol Pharm. 2010 Aug 2;7(4):1057–1068. doi: 10.1021/mp900178j

Table 2.

Primers utilized for PHT qRT-PCR analysis and their relative hCMEC/D3 expression.

Amplicon	Genebank	qRT-PCR Primers		hCMEC/D3 Relative Expression [Mean ΔC_t × 10⁻³ ± (S.D.)]

hPHT1	NM_145648	Sense:	CCAACATCACGCCCTTCG	0.764 (0.05)^†
hPHT1	NM_145648	Antisense:	ACAGTGGGGATCGCATAA	0.764 (0.05)^†

hPHT2	NM_016582	Sense:	TGCTGGTGGTGGCGTTTATTCA	1.021 (0.34)^†
hPHT2	NM_016582	Antisense:	TGGAAGTTGGCGATGTCCTCTT	1.021 (0.34)^†

GAPDH	NM_002046	Sense:	TTCGACAGTCAGCCGCATCTTCTT	N/A
GAPDH	NM_002046	Antisense:	GCCCAATACGACCAAATCCGTTGA	N/A

^†

Relative expression of SLC15A4 and SLC15A3 were determined separate from the SA Biosciences RT² Profiler™ Array, as they are not included on the validated array. Relative expression reported is the mean ΔC_t of three individual replicates, normalized to the housekeeping gene GAPDH.