Skip to main content
. Author manuscript; available in PMC: 2011 Aug 15.
Published in final edited form as: Dev Biol. 2010 Jun 11;344(2):896–910. doi: 10.1016/j.ydbio.2010.06.006

Figure 1. αN-catenin transcripts and protein distribution in the chick embryo midbrain.

Figure 1

(A,C,E,G) Whole-mount in situ hybridization for αN-catenin at various stages of development, with indicated transverse sections (B,D,F,H) for whole-mounts, respectively. (I) Higher magnification image of the dorsal neural tube region found in (H). In (H) and (I), neural tube cells express αN-catenin but newly migratory neural crest cells lack αN-catenin (arrowheads). (J,M,P,S) Immunohistochemistry for αN-catenin protein (green) on transverse sections taken through the chick embryo midbrain, with representative sections shown for specific developmental stages. (K,N,Q,T) DAPI staining of (J,M,P,S) to mark cell nuclei. (L,O,R,U) Merge αN-catenin and DAPI images. (V-X) Magnified field of dorsal neural tube region found at 7ss in (S-U). Arrow (S) and arrowheads (S,V) indicate diminished αN-catenin protein in the apico-dorsal region of the neural tube and the absence of αN-catenin protein on newly migratory neural crest cells, respectively. (Y,Z) Negative control immunohistochemistry experiments for αN-catenin in the absence of primary (αN-catenin) antibody (Y) and secondary antibody (Z). Scale bar in (A) is 20 μm and is applicable to all whole-mount images, while individual scale bars (50 μm) are shown for each section. Scale bar in (J) is 50 μm and applicable to images (J-U,Y,Z). Scale bar for (V-X) is 50 μm and is shown in (V). ss, somite stage.