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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Mol Pharm. 2010 Aug 2;7(4):1283–1290. doi: 10.1021/mp100073s

Figure 4.

Figure 4

A. Real-time PCR to detect Hsp27 mRNA level in UM-SCC-22B cells after siRNA transfection. SiHsp27, transfected with siRNA of Hsp27. Ctrl, cell treated with transfection reagents lipofectamine 2000; Sictrl: cells transfected with scrambled siRNA. B. Western blotting to detect protein levels of Hsp27 in UM-SCC-22B cells after siRNA transfection. The cells were transfected with scrambled siRNA (sictrl) or Hsp27 siRNA (siHsp27) for 48hr. The resulting cells were lysed, and the soluble protein (35ug protein per lane) was separated by SDS-PAGE and detected by western blotting using the specific antibodies. β-actin was used as control for protein loading.