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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Dev Comp Immunol. 2010 Jun 25;34(10):1119–1128. doi: 10.1016/j.dci.2010.06.007

Fig. 1. In vitro proPO activation in M. sexta larval plasma by LPS, LTA and PG.

Fig. 1

(A) Aliquots of naïve plasma were incubated alone, or with LPS-K12, PG-K12, LTA-BS, PG-BS, LTA-SA or PG-SA in wells of a 96-well microtiter plate for 30 min at room temperature, and PO activity was determined as described in the Materials and Methods. Data from four replicates of each sample were analyzed. (B) Aliquots of naïve plasma were incubated at room temperature alone, or with LPS-K12 or LTA-BS for 30, 60, 90 or 120 min, and PO activity was determined. Each assay was performed in four replicates. The bars represent the mean of four individual measurements ± S.E.M. The significance of difference was determined by an unpaired t-test using the GraphPad InStat software. Asterisks (*) indicate significant difference compared to the naïve plasma alone group. Significance between two groups was indicated by a horizontal line and p value was shown above the line.