Table 2.
Pathological lesions and antigen distribution in tissues of dogs infected with H5N1 virus A/bar-headed goose/Qinghai/3/05a.
| Animal | Administration route | Day euthanized post-infection (p.i.) |
Tonsil | Trachea | TBLN | Left lungs | Middle lung | Right lungs | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Upper | Lower | Upper | Middle | Lower | |||||||
| B1 | Intranasal | 3 | +/++ b | −/− | −/− | +/+ | −/− | −/− | −/− | −/− | −/− |
| B2 | Intranasal | 3 | +/++ | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− |
| B3 | Intranasal | 5 | +/+ | −/− | −/− | −/− | −/− | ++/++ | −/− | −/− | −/− |
| B4 | Intranasal | 5 | +/+ | −/− | −/− | ++/+ | +/− | +++/++ | +/− | +/− | ++/+ |
| B7 | Intratracheal | 3 | −/− | −/− | −/− | +/− | +/− | −/− | +++/+++ | +/− | +/− |
| B8 | Intratracheal | 3 | +/++ | −/− | −/− | ++/+ | −/− | −/− | +++/++ | +/− | +/+ |
| B9 | Intratracheal | 5 | −/− | −/− | −/− | +++/++ | +++/++ | +++/++ | +++/++ | ++/++ | ++/+ |
| B10 | Intratracheal | 4c | −/− | −/− | −/− | ++/− | ++/− | +++/++ | +++/+ | +++/+ | +++/+ |
a
Beagles (45 days old) were inoculated intranasally (i.n.) or intrachacheally (i.t.) with 1 ml of 106EID50 of the BHG/QH/3/05 virus. Two animals from each group were euthanized on days 3 and 5 p.i., respectively, and their organs were collected for assessment of pathological lesions and antigen distribution.
b
Pathological lesions/viral antigens. −, no pathological change/antigen. +, limited pathological change/antigen. ++, moderate pathological change/antigen. +++, severe pathological change/abundant antigen. TBLN: tracheobronchial lymph node.
c
This animal died on day 4 p.i.