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. 2010 Jul 1;405(1):139–148. doi: 10.1016/j.virol.2010.05.031

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Copyright © 2010 Elsevier Inc. All rights reserved.

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Fig. 6 — SARS-CoV S palmitoylation is necessary for partitioning into detergent-resistant membranes. At 24 h post-transfection, detergent-resistant membranes (DRMs) were extracted from HEK293T cells expressing SARS-CoV S or S_PN using cold Triton. DRMs were isolated using discontinuous density ultracentrifugation, and fractions were collected from the top. S protein was identified by Western blotting (upper and middle panels) and DRMs were identified using HRP-cholera toxin B, which binds ganglioside GM1 (bottom panel). A representative image of 3 independent experiments is shown.