Skip to main content
. 2010 Jul 23;398(2):254–259. doi: 10.1016/j.bbrc.2010.06.069

Fig. 2.

Fig. 2

Fluorescence energy transfer. (A) Fluorescence emission scans of 200 nM GFP-MTase showing the effects of FRET to 200 nM HEX-labelled DNA. GFP-MTase alone (line), GFP-MTase – 21TH21B DNA complex (dashed line), GFP-MTase – 21T21BH DNA (dotted line). Excitation was at 395 nm. (B) Fluorescence emission scans of 500 nM GFP-MTase showing FRET to 500 nM Dylight549-labelled mutant ocr proteins. GFP-MTase alone (line), GFP-MTase – E20C ocr complex (dashed line), GFP-MTase – S68C ocr complex (dotted line), GFP-MTase – E117C ocr complex (small crosses). Excitation was at 395 nm.