Table 1.
Modification of phage λv.o by GFP-MTase protects the phage DNA against the EcoKI R/M system.
Phage recovered from NM1261(DE3) containing the following plasmids | Strain used for plating of recovered phage | Titre of phage on plating strain (pfu/ml) |
---|---|---|
pBIO2 | NM1261(DE3) r−m− | 3.0 × 108 |
pBIO2 | NM1049(DE3) r+m+ | 1.4 × 104 |
pJFMS | NM1261(DE3) r−m− | 0.8 × 108 |
pJFMS | NM1049(DE3) r+m+ | 1.2 × 108 |
pJFMSEGFP | NM1261(DE3) r−m− | 1.4 × 108 |
pJFMSEGFP | NM1049(DE3) r+m+ | 1.5 × 108 |