Table 2.
Time-resolved fluorescence decay analysis of samples showing FRET between GFP and HEX or Dylight549. Excitation at 405 nm, emission at 510 nm. The pre-exponential factor for each lifetime is given in the brackets.
| Sample | τ1(ns) | τ2(ns) | τ3(ns) | χ2 | <τ>(ns) |
|---|---|---|---|---|---|
| GFP-MTase | 2.20 (0.36) | 3.01 (0.64) | 1.082 | 2.72 | |
| GFP-MTase + 21TH21B DNA | 0.29 (0.38) | 1.42 (0.29) | 2.84 (0.33) | 1.076 | 1.45 |
| GFP-MTase + 21T21BH DNA | 1.61 (0.31) | 2.84 (0.69) | 1.061 | 2.46 | |
| GFP-MTase + E20C ocr | 0.28 (0.27) | 1.42 (0.40) | 2.72 (0.33) | 1.052 | 1.55 |
| GFP-MTase + S68C ocr | 1.37 (0.44) | 2.67 (0.56) | 1.173 | 2.09 | |
| GFP-MTase + E117C ocr | 0.33 (0.38) | 1.28 (0.32) | 2.84 (0.29) | 1.012 | 1.38 |