Table 3.
Steady-state kinetic parameters with respect to isochorismate
| Protein | Km (μM) | kcat (min− 1) | kcat/Km (μM− 1 min− 1) | Relative kcat/Km |
|---|---|---|---|---|
| Wild type | 1.1 ± 0.2 | 19 ± 1 | 19 ± 3 | 1 |
| R409A | 8.5 ± 1.2 | 1.4 ± 0.1 | 0.16 ± 0.02 | 0.009 |
| R428A | 1.1 ± 0.5 | 2.8 ± 0.8 | 2.8 ± 0.8 | 0.15 |
| R32A′ | 49 ± 7 | 11 ± 1 | 0.22 ± 0.01 | 0.01 |
| R106A′ | 41 ± 4 | 0.7 ± 0.2 | 0.018 ± 0.01 | 0.00095 |
| K299A | 8.4 ± 1.2 | 25 ± 2 | 3.0 ± 0.3 | 0.16 |
| L493A | 39 ± 1 | 19 ± 1 | 0.49 ± 0.03 | 0.03 |
| I489A | 27 ± 1 | 5.7 ± 0.2 | 0.21 ± 0.01 | 0.01 |
| F490 | 29 ± 3 | 1.7 ± 0.1 | 0.058 ± 0.01 | 0.003 |
Residues marked with ′ are located in the second subunit forming the active site.