Table 2.
Sequences of the primers and the TflijrMan-probea
| Primer name | Origin | Sequence |
|---|---|---|
| Primer F (forward) | Corless et al., 2000 [4] | 5′-CCATGAAGTCGGAATCGCTAG-3′ |
| Primer T (reverse) | Corless et al., 2000 [4] | 3′-ACTCCCATGGTGTGACGG-3′ |
| Primer 16S-Mtb-F (forward) | This work | 5′-CCGTGAAGTCGGAGTCGCTAG-3′ |
| Primer 16S-Mtb-R (reverse) | This work | 5′-CCACTTTCATGACGTGACGG-3′ |
| Primer 16S-Staph-F (forward) | This work | 5′-CCATGAAGCTGGAATCGCTAG-3′ |
| Primer 16S-Staph-R (reverse) | This work | 5′-CACTCTCGTGGTGTGACGG-3′ |
| Probe | Corless et al., 2000 [4] | FAM-5′-CGGTGAATACGTTCCCGGGCCTTGTAC-3′-TAMRA |
a
The TaqMan probe was marked with 6-carboxyfluorescein (FAM) at the 5′-terminus and with 6-carboxytetramethylrhodamine (TAMRA) at the 3′-terminus.