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. 2010 Jan 7;37(1):21–28. doi: 10.1159/000265571

Table 3.

Bacterial DNA decontamination trial of Taq polymerase using the restriction enzyme Sau 3 Ala

Untreated Sau 3 Al
1:3 1:6
E. coli (10,000 CFU) 24.51 30.95 33.26
Negative control (elution buffer) 32.96 38.62 39.68
Delta C(t) negative control – E. coli 8.45 7.67 6.42
a

C(t) values of untreated and mixing ratios of TaqMan Universal PCR Master Mix to Sau 3A1 of 1 unit : 3 units restriction enzyme and 1:6 ratio are shown.