Table 3.
Bacterial DNA decontamination trial of Taq polymerase using the restriction enzyme Sau 3 Ala
Untreated |
Sau 3 Al |
||
---|---|---|---|
1:3 | 1:6 | ||
E. coli (10,000 CFU) | 24.51 | 30.95 | 33.26 |
Negative control (elution buffer) | 32.96 | 38.62 | 39.68 |
Delta C(t) negative control – E. coli | 8.45 | 7.67 | 6.42 |
C(t) values of untreated and mixing ratios of TaqMan Universal PCR Master Mix to Sau 3A1 of 1 unit : 3 units restriction enzyme and 1:6 ratio are shown.