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Dual effect of neutrophils on SC secretion. Confluent monolayers of Calu-3 cells were incubated in triplicates for 48 hrs with increasing numbers of neutrophils, from 0.3 × 106 to 15 × 106 cells. (a) SC concentration and (b) elastase activity were measured in supernatants, as described in Methods. Data are means ± SEM (n = 6 independent experiments). #P = .08, *P ≤ .05 and **P ≤ .01 as compared with control.
