Figure 6.
Effect of MAP kinase inhibitors on pIgR/SC production. (a) Calu-3 cells were cultured with TGF-β1 (20 ng/ml) for 5 to 120 min and phosphorylation of ERK1/2 and p38 was assayed by western blot, as compared to β-actin as loading control. (b) Calu-3 cells were cultured with TGF-β1 (20 ng/ml) or neutrophils (5 × 106 cells) for 48 hrs in the presence of medium alone (control) or MAP Kinase inhibitors: PD98059 (grey bars), SB203580 (solid bars) or SP600125 (stripped bars) (all 50 μM) as selective inhibitors of ERK1/2 MAPK, p38 MAPK and JNK MAPK, respectively. SC production was assessed in supernatants and results were shown as means ± SEM (n = 4). *P < .05, **P < .01 as compared with control.