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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Mol Cell. 2010 Jun 25;38(6):781–788. doi: 10.1016/j.molcel.2010.06.001

Figure 1.

Figure 1

Validation of bioinformatically predicted miRNA cleavage targets by 5′ RACE in 293S cells. (A) Nested PCR of the 5′ RACE produces amplicons of the expected size for ATPAF1 and LYPD3, but not a negative control (lane 3). (B) Cloning and sequencing of the PCR amplicons indicates products corresponding to the expected miRNA cleavage site. (C) and (D) Conservation of the mir-151-5p target site in the 3′ UTRs of ATPAF1 and LYPD3. See also Supplementary Table S1.