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. Author manuscript; available in PMC: 2010 Oct 22.
Published in final edited form as: Nature. 2010 Apr 4;464(7292):1196–1200. doi: 10.1038/nature08889
Morpholino sequences
klf2a ATG MO GGACCTGTCCAGTTCATCCTTCCAC
klf2a splice blocking MO CTCGCCTATGAAAGAAGAGAGGATT
miR-126 MO TGCATTATTACTCACGGTACGAGTTTGAGTC
spred1 MO AAACCTGTGGAAGGAGAAGGAAACC
control MO Oligo-31N
tnnt2 MO CATGTTTGCTCTGATCTGACACGCA
Primers
Klf2a attb1 gggg aca agt ttg tacaaaaaagcaggctacaggtcc ATGCATCTCAGC
Klf2a attb2 GGGGACCACTTTGTACAAGAAAGCTGGGTcattttccagagtccgttcC
Spred 3′UTR attb2 GGGG ACA GCT TTC TTG TAC AAA GTG G
CGCTTGTGCCACCGCTGC
Spred 3′UTR attb3 GGGG AC AAC TTTGTATAATAA AGT TG
GCAGAGCGTTTGGCGGTGT
PIk3r2 3UTR attb2 GGGG ACA GCT TTC TTG TAC AAA GTG G
CACAACGACTCGCTGAATGT
PIK3r2 3UTR attb3 GGGG AC AAC TTT GTA TAA TAA AGT TG
TCCTCAAGCTGGGATCATGT
Spred ORF attb1 ggggacaagtttgtacaaaaaagcaggctacatgagcgaa gaa cca aac
Spred ORF attb2 ggggaccactttgtacaagaaagctgggttcatcctgcagccttgt
miR126 chm8 F TGCCATGCCTTGACGAGAAG
miR126 chm8 R GTGATATTTAATGTAACATGCC
qpcr Bact F AGCTTGAAACTCGCCAAGTG
qpcr Bact R CAGCTTTATAGCCGGCACTG
qpcr vegf F GCCAAAGGCAGAAGTCAAAG
qpcr vegf R TGCAGGAGCATTTACAGGTG
qpcr Kdrl F TCTACTGGGCTTTTCCCTCTC
qpcr Kdrl R AGGGTTACTATGGTGACGTTGC
qpcr Zelastin F GCTCGTCTCCATACAAAGCA
qpcr Zelastin R CAGAACTCCTCCTGGTAGCC
qpcr Z Tie2 F GCCGTCAAGAGGATGAAAGA
qpcr Z Tie2 R GCTGCTGTGAGGAGAGTGTG
qpcr Znos1 F TCAAATACGCCACCAACAAA
qpcr Znos1 R GAGAAGAAGGGGCAAAACATC
qpcr ZItgb5 F TGGGAAGGATGGACAAAGAG
qpcr ZItgb5 R CGGGTGAATGAGGAGACACT
qpcr ZEdg1 F CCACCGTATTCAGCGTTATT
qpcr ZEdg1 R GTCAAGGAGGAGCAGGATGA
qpcr ZEdn 1 F ATGAGGCGAAACCACAGAAG
qpcr ZEdn 1 R AGAAACCACTTGAGCGAGG
F-qpcr klf2a CCAACGCCAACCAAAGAGTA
R-qpcr klf2a CTCGCCTGTGTGTGTTCTGT
Mir 126 qpcr F for miRscript CGT ACC GTG AGT AAT AAT GC
R-RT-PCR klf2a ACGTGGTACCCGCACGGCGAACTCACACTTG
F-RT-PCR klf2a ACGTGGTACCacgcacggcgactcacacttg
miRNA Duplex
miR-126 sense ucguaccgugaguaauaaugc
miR-126 antisense gcauuauuacucacgguacga
miR-126 scramble antisense gcaccacaacucaagguucga