Table 2.
Stability of topotecan lactone in lysed human whole blood.
| Nominal Concentration (ng/mL) | Storage condition | Measured Concentration. (ng/mL)a | Accuracy (%)b |
|---|---|---|---|
| 7.00 | 0 h (Baseline) | 6.5 | |
| RT (3 h) | 3.18 | 49.3 | |
| 4°C (3 h) | 6.04 | 93.6 | |
| day 0 (Baseline) | 6.80 | ||
| −80°C (28 days) | 5.98 | 87.9 | |
| 2 F-T | 6.77 | 99.6 | |
| 80.00 | 0 h (Baseline) | 76.2 | |
| RT (3 h) | 40.0 | 52.5 | |
| 4°C (3 h) | 70.4 | 92.4 | |
| day 0 (Baseline) | 78.6 | ||
| −80°C (28 days) | 73.9 | 94.0 | |
| 2 F-T | 76.9 | 97.8 |
F-T= freeze thaw-cycle
RT=Room (ambient) temperature
a
Average (n=2)
b
(Average measured concentration/average measured concentration at t=0) × 100