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. Author manuscript; available in PMC: 2011 Jul 28.
Published in final edited form as: J Am Chem Soc. 2010 Jul 28;132(29):9944–9947. doi: 10.1021/ja103983f

Figure 1.

Figure 1

A series of 15N chemical shift spectra acquired using ramp-CP and RINEPT (A), and DREPT (B) experiments on magnetically-aligned bicelles containing a uniformly-15N-labeled mutant-cytochrome-b5. The contact time used for ramp-CP was 800 μs. The delay periods used in RINEPT were τ1 = τ2 = 2ms. The refocusing delay (τ2) in the DREPT sequence used to obtain each spectra in (B) is indicated and in all these cases τ1 = τ2. All the spectra were acquired using 2000 scans and a 3s recycle delay. (C and D) 2D 1H-15N HETCOR spectra of the same sample obtained using the DREPT pulse sequence (Figure S1). These spectra were acquired using 20 t1 increments, 800 scans per increment, and a 3s recycle delay. The delay τ2 period used in these HETCOR spectra was 1ms for (C) and 80 μs for (D).