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. Author manuscript; available in PMC: 2010 Aug 3.
Published in final edited form as: Prostate. 2007 Dec 1;67(16):1801–1815. doi: 10.1002/pros.20662

Fig. 4.

Fig. 4

Comparison of the effects of anti-AR shRNA and siRNA transfection with and without androgen withdrawal on AR-dependent reporter activity. LNCaP (A) and CWR22R (B) cells were cotransfected with either shRNA vectors (shAR1 or shGFP) or siRNA mixtures from Dharmacon (AR or GAPDH) together with the ARE-Luc reporter. Twenty-four hours after transfection cells were split and transferred to either FBS- or CSS-containing media. After an additional 24-hr incubation, luciferase activity was measured. Reporter activity is shown relative to that of cells transfected with shGFP and kept in FBS-containing medium (set at 1.0).