Fig. 5.

Inhibition of AR expression in CWR22R cells leads to cell death through apoptosis. CWR22R cells were transfected with siRNA mixtures targeting either AR or GAPDH or left untransfected (mock). siGLO (Dharmacon) was added to monitor transfection efficiency. Experiment was done in standard FBS containing medium. A: Western blot of cell lysates 48 hr after transfection probed with anti-AR antibody. Bands corresponding to the two polypeptides of the AR present in CWR22R cells [38] (110 and 80 kDa) are indicated by arrows. B: Western blots of cell lysates collected 4 or 6 days after transfection probed with anti-AR, anti-caspase 3, and anti-PARP antibodies. Cleavage of the 35 kDa Caspase 3 protein into 17 and 19 kDa fragments and of the 116 kDa PARP protein into an 89 kDa product is shown. C: FACS analysis of cells fixed and stained with propidium iodide 4 or 6 days after transfection.