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. Author manuscript; available in PMC: 2010 Aug 3.
Published in final edited form as: Prostate. 2007 Dec 1;67(16):1801–1815. doi: 10.1002/pros.20662

Fig. 6.

Fig. 6

CaP cells do not tolerate overexpression of AR. A: Photographs of methylene blue-stained plates of cells. CWR22R (expressing endogenous mutant AR), LNCaP (expressing endogenous mutant AR), and PC3 (AR-null) cells were transfected with either AR cDNA or empty vector and selected in media containing hygromycin for 14 days. Transfection efficiency was normalized by cotransfection with pCMV-LacZ. B: Selected colonies that survived hygromycin selection following transfection with vector (v) or AR cDNA (AR) were expanded and analyzed by Western blot for AR protein levels.