Table 2.
Peak volumes for simulated LC×LC chromatograms to evaluate changes in sampling phase using the moments method.a
| 1σ = 0.12 min; MR= 1.37 | First Dimension Retention Time (min) | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Slice Position (min) | 1.050 | 1.075 | 1.100 | 1.125 | 1.150 | 1.175 | 1.200 | 1.225 | |
| Slice # | Sampling phase (φ) | 0 | −0.143π | −0.286π | −0.428π | −0.572π | −0.714π | −0.856π | −π |
| 3 | Area @ 0.70 min | 5.8 (12)b | 3.8 (6.9) | 2.4 (5.3) | 1.5 (3.9) | 0.9 (1.7) | 0.5 (1.1) | 0.3 (0.6) | 0.2 (0.4) |
| 4 | Area @ 1.05 min | 68.4 (130) | 67.7 (140) | 65.6 (150) | 62.3 (170) | 57.9 (130) | 52.4 (100) | 46.3 (85) | 39.8 (100) |
| 5 | Area @ 1.40 min | 5.8 (14) | 8.4 (17) | 11.9 (19) | 16.2 (25) | 21.3 (43) | 27.0 (71) | 33.3 (69) | 39.8 (82) |
| 6 | Area @ 1.75 min | 0.0 (0.2) | 0.0 (0.2) | 0.0 (0.2) | 0.0 (0.2) | 0.1 (0.2) | 0.1 (0.2) | 0.1 (0.2) | 0.1 (0.4) |
| Sum of All Areasc | 80.0 | 79.9 | 80.0 | 80.1 | 80.0 | 80.0 | 80.0 | 80.0 | |
| 1σ = 0.20 min; MR = 2.29 | First Dimension Retention Time (min) | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Slice Position (min) | 1.050 | 1.075 | 1.100 | 1.125 | 1.150 | 1.175 | 1.200 | 1.225 | |
| Slice # | Sampling phase (φ) | 0 | −0.143π | −0.286π | −0.428π | −0.572π | −0.714π | −0.856π | −π |
| 3 | Area @ 0.70 min | 14.9 (35) | 12.5 (27) | 10.2 (18) | 8.3 (18) | 6.7 (17) | 5.3 (15) | 4.2 (8.8) | 3.2 (7.0) |
| 4 | Area @ 1.05 min | 49.5 (93) | 49.2 (110) | 48.3 (110) | 46.9 (89) | 45.0 (110) | 42.5 (80) | 39.8 (88) | 36.8 (93) |
| 5 | Area @ 1.40 min | 14.9 (28) | 17.6 (26) | 20.6 (51) | 23.7 (58) | 26.9 (60) | 30.3 (67) | 33.6 (61) | 36.8 (93) |
| 6 | Area @ 1.75 min | 0.0 (0.7) | 0.5 (1.0) | 0.7 (1.8) | 1.0 (1.9) | 1.4 (3.0) | 1.8 (3.4) | 2.4 (4.9) | 3.2 (7.8) |
| Sum of All Areasc | 79.3 | 80.1 | 79.9 | 80.0 | 80.0 | 80.1 | 80.1 | 80.1 | |
a
See Fig. 1 to interpret data. All data are the average of the peak areas for 500 runs at the indicated slice position for the indicated first dimension retention time.
b
Number in parentheses is the signal-to-noise of the peak.
c
This is the sum of the above peak areas. The true volume is 80.0.