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. Author manuscript; available in PMC: 2011 Jul 23.
Published in final edited form as: J Mol Biol. 2010 May 24;400(4):755–767. doi: 10.1016/j.jmb.2010.05.044

Figure 2.

Figure 2

The V67L mutation causes global changes, affecting the N- and C-terminus of the intein. Peaks with large chemical shift perturbations are labeled. (a) Overlay of 15N-1H HSQC spectra of ΔΔIhh-V67CM (cyan) and ΔΔIhh-L67CM (red) mutants. (b) Chemical shift difference Δδ=(10ΔδH)2+(ΔδN)2 between ΔΔIhh-V67CM and ΔΔIhh-L67CM mutants plotted against residue number. (c) Projection of V67L chemical shift perturbation onto the crystal structure ofΔΔIhh-L67CM (pdb code: 2IN8). The ribbon color is linearly interpolated between red and purple for Δδ between 3.0 and 1.0 ppm; and between purple and cyan for Δδ between 1.0 and 0 ppm. The ribbon is colored grey when chemical shift differences are not available, for prolines, N-terminal residue and unassigned residues. Residue L67 is displayed in meshed CPK mode.