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. Author manuscript; available in PMC: 2010 Aug 3.
Published in final edited form as: J Nat Prod. 2007 Oct 25;70(11):1741–1745. doi: 10.1021/np070206e

Figure 3.

Figure 3

Effects of 4 on the induction of secreted VEGF protein. Exponentially grown T47D cells were exposed to 4 under hypoxic conditions (1% O2) or in the presence of 1,10-phenanthroline (10 μM) for 16 h. The level of secreted VEGF protein in the conditioned media was determined by ELISA and normalized to the level of protein in cellular lysates. Data shown are averages from one experiment performed in triplicate and the bars represent standard deviation. Data were compared by ANOVA followed by Dunnett’s multiple comparison test using GraphPad Prism 4 software. The p-values between the induced control and 4 treated samples are shown.