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. Author manuscript; available in PMC: 2010 Aug 3.
Published in final edited form as: Cell. 2008 May 16;133(4):612–626. doi: 10.1016/j.cell.2008.03.025

Figure 7. Cell Growth Repression Function of p53 Is Abrogated by Loss of Acetylation but Rescued by siRNA-Mediated Ablation of Mdm2 and Mdmx.

Figure 7

(A) Tet-off-p53 cells and Tet-off-p53-8KR cells treated with control siRNA, Mdm2 siRNA, Mdmx siRNA, or a combination of Mdm2 siRNA and Mdmx siRNA, were induced by removal of tetracycline from the media. The total cell extracts were analyzed by western blot using antibodies against p53 (DO-1), Mdmx, Mdm2, Pig3, p21, and actin.

(B) In the upper panel, p53-8KR is unable to induce cell death and growth arrest. Tet-off-p53 and Tet-off-p53-8KR cells were plated at 10% confluence and incubated in the presence (uninduced) or absence (induced) of tetracycline for 6 days. The plates were washed with PBS and stained with crystal violet. In the lower panel, Tet-off-p53-8KR treated with either control siRNA or Mdm2/Mdmx siRNA were split and incubated in the presence (uninduced) or absence (induced) of tetracycline for 6 days. The plates were stained with crystal violet.