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Immunofluorescence assay of SEPT7 (green) and acrosome (Lectin, red) in developing spermatozoa. a, b: Round spermatids; c, d: Elongating spermatids; e, f: Elongated spermatids; g, h: mature spermatozoa. SEPT7 migrates from the cytoplasm (concentrated in the perinuclear region) to the annulus during sperm maturation. a, c, e and g: merged pictures for SEPT7 and Lectin; b, d, f and h: merged pictures for SEPT7, Lectin and DAPI. The nucleus was stained with DAPI (blue)
