Table 2.
Primers used to amplify specific region of FSH receptor gene. PCR products were used for restriction fragment length polymorphism, single-strand conformation polymorphism or DNA sequencing
| Region of FSH receptor gene | Primer No. | Primer sequence | Product size (bp) | Primer location | Reference No. |
|---|---|---|---|---|---|
| Promoter | 1F | 5′ TATTCCAGACATGCCTAATGG 3′ | 291 | −263 to +28 | Wunsch et al. 2005 [26] |
| 1R | 5′ CCAGCAAAGAGACCAGGAGC 3′ | ||||
| Exon 1 | 2F | 5′ GGAGCTTCTGAGATCTGTGG C 3′ | 235 | −71 to intronic | Gromoll et al. 2000 [25] |
| 2R | 5′ AAATGCCAGCCATGCAGTTG 3′ | ||||
| Exon 2 | 3F | 5′ TCACTAGTGTAGACAGGATG 3′ | 252 | intronic | Gromoll et al. 2000 [25] |
| 3R | 5′ TTGAGGCATTCACTCACAGC 3′ | ||||
| Exon 3 | 4F | 5′ GCCCTAAAATATTCAAGAG 3′ | 215 | intronic | Gromoll et al. 2000 [25] |
| 4R | 5′ GCCTCCCAGGAATGTAGAA 3′ | ||||
| Exon 4 | 5F | 5′ GCATTCCTTACCATCAAGATC 3′ | 152 | intronic | Gromoll et al. 2000 [25] |
| 5R | 5′ GTGGGGGTACCAAACTACATG 3′ | ||||
| Exon 5 | 6F | 5′ GAAATTCATGCTCTAACAACTG 3′ | 200 | intronic | Gromoll et al. 2000 [25] |
| 6R | 5′ TGGGCAAGACAGATACTGAG 3′ | ||||
| Exon 6 | 7F | 5′ ACATTCAAGATAACATAAAC3′ | 101 | intronic | Present study |
| 7R | 5′ GCGGATCCTTACAGAATCACACTTTC 3′ | ||||
| Exon 7 | 8F | 5′ TTCAGATGGCTGAATAAGA 3′ | 86 | intronic to 655 | Present study |
| 8R | 5′ GCGGATCCGCTCATCTAGTTGGGTTC3′ | ||||
| Exon 8 | 9F | 5′ TACAACAGCAATATTTATGTGGC 3′ | 198 | intronic | Gromoll et al. 2000 [25] |
| 9R | 5′ GAGAGTTGACTTCTAACTTACAC 3′ | ||||
| Exon 9 | 10F | 5′ TGCCTGCTAACCAAGAGCAG 3′ | 289 | intronic | Gromoll et al. 2000 [25] |
| 10R | 5′ TGCCTGAGCAGGGCTTAAAG 3′ | ||||
| Exon 10A | 11F | 5′ GCTATACTGGATCTGAGATG 3′ | 221 | intronic to 1026 | Gromoll et al. 2000 [25] |
| 11R | 5′ ACCACTTCATTGCATAAGTC 3′ | ||||
| Exon 10B | 12F | 5′ TTGACATGACGTACACTGAG 3′ | 289 | 976 to 1264 | Gromoll et al. 2000 [25] |
| 12R | 5′ CAACTGATGCAATGAGCAG 3′ | ||||
| Exon 10C | 13F | 5′ CTGATCTCTGCATTGGAATC 3′ | 265 | 1219 to 1483 | Gromoll et al. 2000 [25] |
| 13R | 5′ ATCCAGCCCATCACCATGAC 3′ | ||||
| Exon 10D | 14F | 5′ AGCTGGACTGCAAGGTGCAG 3′ | 260 | 1426 to 1674 | Gromoll et al. 2000 [25] |
| 14R | 5′ GGTTCCGCACTGTGAGGTAG 3′ | ||||
| Exon 10E | 15F | 5′ CCTTGTGCTCAATGTCCTGG 3′ | 219 | 1601 to 1820 | Gromoll et al. 2000 [25] |
| 15R | 5′ GCTTTGGACACAGTGATGAG 3′ | ||||
| Exon 10F | 16F | 5′ CCATTTCTGCCTCCCTCAAG 3′ | 227 | 1722 to 2000 | Gromoll et al. 2000 [25] |
| 16R | 5′ TGGATGGGTGTTGTGGACAG 3′ | ||||
| Exon 10G | 17F | 5′ GAGCAAGTGTGGCTGCTATG 3′ | 258 | 1922 to −91(3′ region) | Gromoll et al. 2000 [25] |
| 17R | 5′ TGTAGAAGCACTGTCAGCTC 3′ | ||||
| Exon 10 | 18F | 5′ AGCTCTGAGCTTCATCC 3′ | 357 | 856 to 1209 | Present study |
| 18R | 5′ GTTGCACATAAGGAACC 3′ | ||||
| Exon 10 | 19F | 5′ CCCATGTGAAGATATCATG 3′ | 328 | 1061 to 1389 | Present study |
| 19R | 5′ GATAGCTGTCGGAGTG 3′ | ||||
| Exon 10 | 20F | 5′ GGCCTTTGCTGATCCCTG 3′ | 868 | 1211 to 2084 | Present study |
| 20R | 5′ GGGCTAAATGACTTAGAGGG 3′ |