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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Mol Microbiol. 2010 Apr 1;76(4):918–931. doi: 10.1111/j.1365-2958.2010.07144.x

Figure 5. YplA secretion assays.

Figure 5

Secretion of flagellin-YplA fusion proteins from Y. enterocolitica. The 5′ end of the flaA or flaB genes, gene. Panels: A) which encode the first 36 residues, were fused to the truncated yplA Secretion assay performed using Y. enterocolitica yplA mutant harboring pCSP50 empty vector; B) Secretion assay performed using Y. enterocolitica yplA mutant harboring flaA-yplA; C) Secretion assay performed using Y. enterocolitica yplA mutant harboring flaB-yplA; and D) Relative secretion efficiency of Y. enterocolitica yplA mutant harboring modified flaA-yplA and flaB-yplA fusion gene constructs. The values shown represent the mean zone of secretion from six individual assays, and the error bars represent ± one standard deviation. The width of the zone of secretion was determined by measuring from the edge of colony growth to the outer edge of precipitation. A standard curve was created by assaying varying combinations of pCSP50:flaA and pCSP50 containing Y. enterocolitica.