Figure 3.
Expression of factor H–binding protein (fHbp) in outer membrane vesicle (OMV) preparations of representative isolates, as measured by Western blot analysis. Monoclonal antibodies (MAbs) JAR 1 and JAR 5 were raised against fHbp variant 1 (v.1) (gene from strain MC58); MAb JAR 31 was raised against fHbp variant 3 (v.3) (gene from strain M1239), but it cross-reacts with fHbp variant 2 (v.2) and v.3 proteins. OMV from control strains included MC58 wt (fHbp v.1), MC58 fHbp knockout (ΔfHbp) mutant, and RM1090 WT (fHbp v.2). The sequence type (ST) of each isolate is shown in parentheses. rfHbp, recombinant fHbp.