Table I.

rhIL-7 treatment increases T cell absolute numbers, activated T cells and Bcl-2 expression in different T cell subsets

	Naive			Central Memory			Effector Memory
	CD4 T Cells
	× 106	Bcl-2 MFI	CD69+	× 106	Bcl-2 MFI	CD69+	× 106	Bcl-2 MFI	CD69+
Sham	5.6 ± 0.1	1398 ± 110	0.18 ± 0.01	0.74 ± 0.04	1233 ± 68	0.09 ± 0.01	0.89 ± 0.02	913 ± 59	0.16 ± 0.01
Sham + IL7	6.6 ± 0.2	1729 ± 126	0.18 ± 0.01	0.91 ± 0.06	1607 ± 28	0.12 ± 0.01	1.0 ± 0.06	1147 ± 62	0.19 ± 0.02
p Valuea	<0.01				<0.01	<0.05		<0.05
CLP	3.6 ± 0.3	1271 ± 13	0.32 ± 0.03	0.91 ± 0.1	1168 ± 38	0.26 ± 0.04	0.49 ± 0.03	812 ± 19	0.17 ± 0.02
CLP + IL7	5.7 ± 0.5	1376 ± 11	0.42 ± 0.04	1.29 ± 0.2	1498 ± 38	0.30 ± 0.04	0.72 ± 0.04	1029 ± 30	0.26 ± 0.02
p Valuea	<0.001	<0.01	<0.05	<0.05	<0.01		<0.001	<0.01	<0.01
	CD8 T Cells
Sham	3.5 ± 0.1	1804 ± 115	0.11 ± 0.002	1.7 ± 0.22	2668 ± 216	0.07 ± 0.01	0.14 ± 0.01	1732 ± 116	0.01 ± 0.002
Sh + IL7	4.6 ± 0.3	2435 ± 137	0.13 ± 0.009	2.0 ± 0.04	3411 ± 187	0.1 ± 0.01	0.12 ± 0.01	1966 ± 94	0.01 ± 0.003
p Valuea	<0.05	<0.05
CLP	3.3 ± 0.3	1498 ± 23	0.19 ± 0.02	1.23 ± 0.1	2340 ± 26	0.33 ± 0.06	0.21 ± 0.02	1634 ± 75	0.05 ± 0.01
CLP+ IL7	5.0 ± 0.4	1824 ± 37	0.29 ± 0.03	2.00 ± 0.1	2573 ± 30	0.44 ± 0.06	0.25 ± 0.03	2121 ± 111	0.05 ± 0.01
p Valuea	<0.01	<0.001	<0.05	<0.001	<0.001			<0.05

Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7. Splenocytes were harvested 24 h later, enumerated, and analyzed for CD4, CD8, CD44, CD62L, CD69, and intracellular Bcl-2 by flow cytometry (n = 5 for sham and sham + rhIL-7; n = 8 for CLP and CLP + rhIL-7).

^aDetermined by Student t test.