Fig. 1.

The effect of anginex and Avastin (bevacizumab) on global tumor pO₂ over time. A, global tumor pO₂ is transiently increased by anginex and Avastin in MA148, B16F10, and SCK tumors as measured by Eppendorf pO₂ histograph. -■-, control; -●-, anginex; -▲-, Avastin. Points, average median pO₂ value derived from, on average, 10 values per track, 4 tracks per mouse and 3 to 7 mice per treatment group per day (n = 120-700); bars, SE. B, typical pimonidazole stainings of control, anginex, and Avastin treatment of SCK tumors on days 2 and 7. Representative images of the means for the amount of staining across each section. Original magnification, ×200; bar, 50 μm. C, quantification of pimonidazole stainings of SCK tumors on days 2 and 7 by morphometric analysis. Following binarization of images, hypoxia was estimated by scoring the total number of white pixels per field. Columns, mean black pixel count per image; bars, SE.