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. 2010 Aug 3;4(8):e778. doi: 10.1371/journal.pntd.0000778

Figure 6. Uveitic eye fluids contain antibody to alpha-crystallin B, vimentin and β-crystallin B2.

Figure 6

ELISA results showing significantly higher antibody levels of alpha-crystallin B (A), vimentin (B) and β-crystallin B2-antibodies (C) in uveitic eye fluids. ELISA plate wells were coated with 200 ng of each eye protein. After blocking, wells were sequentially incubated with uveitic or normal eye fluids diluted 1∶100 and HRP-conjugated protein G (1∶5000). Plates were developed using 3,3′,5,5′-tetramethyl benzidine substrate solution and absorbance measured at 450 nm. Presented data is a representative of at least two independent experiments with 3 or more repeats. Error bars indicate standard deviation.