Figure 1.

Changes in the fluorescence intensity of substrates a–e (200 µM) with 23 picomole of (A) Cath E (B) Cath D in 50 mM NaOAc buffer containing 150 mM NaCl (pH 4.0). Solid filled and unfilled markers denote the enzyme treated and untreated substrates, respectively. Without enzymes, fluorescence intensities of all tested substrates remain at the base line level. Values represent the mean of triplicate measurements.