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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Jun 19;1800(9):1002–1008. doi: 10.1016/j.bbagen.2010.06.005

Figure 3.

Figure 3

(A) Structure of intramolecular quenched substrate e, Mca-Ala-Gly-Phe-Ser-Leu**Pro-Ala-Lys(Dnp)-D-Arg-CONH2; (B) Change in the fluorescence intensity of substrate e (200 µM) during the incubation with 23 picomole of Cath E, Cath D, Cath S and Cath B in 50 mM NaOAc buffer of pH 4.0 for Cath E and D and 100 mM NaOAc buffer of pH 6.5 for Cath S and Cath B. Values represent the mean of at least three independent experiments. Error bars represent the upper and lower values of the Standard Error Mean (SEM). Asterisks represent the statistical significance of the two tailed P-values (***, P ≤ 0.001).